Summary
Type: SPE
Application #: 305360
Catalog#: 730747 - SPE Cartridges, Chromabond, HR-XAW, 3mL, 60mg, 30/pk
Detection:
Conditions: Column conditioning: 3 ml methanol, then 3 ml water
(Do not let run the column dry!)
Sample aspiration: 1 ml spiked serum (1 µg/ml)
Washing: 1 ml 2 % formic acid in water
1 ml methanol
Drying: With nitrogen or air
Elution A: 3 ml 5 % ammonia in methanol
- or -
Elution B: 3 ml 5 % formic acid in methanol
Evaporation and reconstitution in suitable solvent
Authors: SPE department
Source: Macherey-Nagel, Germany 2010